Peer-reviewed original research articles

RPE cells engulfmicrovesicles secreted by degenerating rod photoreceptors. Ropelewski P and Imanishi Y eNeuro 6 May 2020, ENEURO.0507-19.2020;DOI:

Disrupted plasmamembrane protein homeostasis in a Xenopus laevis model of retinitis pigmentosa.Ropelewski P and Imanishi Y J Neurosci. 2019 Jul 10;39(28):5581-5593.doi: 10.1523/JNEUROSCI.3025-18.2019.

Müllerglia phagocytose dead photoreceptor cellsin a mouse model of retinal degenerativedisease. Sakami S, Imanishi Y*, PalczewskiK*. The FASEB Journal. 2019 Mar;33(3):3680-3692. (* correspondingauthors)

A small molecule mitigates hearing loss in a mouse modelof Usher syndrome III. Alagramam KN*, Gopal SR, Geng R, Chen DH, Nemet I, Lee R, Tian G, Miyagi M,Malagu KF, Lock CJ, Esmieu WR, Owens AP, Lindsay NA, Ouwehand K, Albertus F,Fischer DF, Bürli RW, MacLeod AM, Harte WE, Palczewski K, Imanishi Y*. Nat Chem Biol. 2016 Apr 25. doi: 10.1038/nchembio.2069. (* corresponding authors) (Featured by “News and Views” of the journal)

Impairment of vision in a mouse model of Usher Syndrome type III.  Tian G, Lee R, Ropelewski P, Imanishi Y. Invest Ophthalmol Vis Sci. 2016 Mar 1;57(3):866-75. doi: 10.1167/iovs.15-16946.

Retinol dehydrogenase8 and ATP-binding cassette transporter 4 modulate dark adaptation of M-cones inmammalian retina. Kolesnikov AV, Maeda A, Tang PH, Imanishi Y, Palczewski K, Kefalov VJ. J Physiol. 2015 Sep 9. doi:10.1113/JP271285. [Epub ahead of print]

Organization of cGMPsensing structures on the rod photoreceptor outer segment plasma membrane.  Nemet I, Tian G, Imanishi Y. Channels (Austin). 2014;8(6):528-35. PubMed PMID:25616687. (Invited research article, Featured by “News and Views” of the journal)

Retrograde intraciliary trafficking of opsin during the maintenance of cone-shaped photoreceptor outer segments of Xenopus laevis.  Tian G, Lodowski KH, Lee R, Imanishi Y. J Comp Neurol. 2014 Nov1;522(16):3577-89. Featured on the cover

Submembrane Assembly and Renewal of Rod Photoreceptor cGMP-Gated Channel: Insight into the Actin-Dependent Process of Outer Segment Morphogenesis. Nemet I, Tian G, Imanishi Y. J Neurosci. 2014 Jun11;34(24):8164-74. Selected as a Featured Article in the Journal of Neuroscience

An unconventional secretory pathway mediates the cilia targeting of peripherin/rds.  Tian G, Ropelewski P, Nemet I, Lee R, LodowskiKH, and Imanishi Y. J. Neurosci.2014 Jan 15;34 992-1006  Recommended by F1000Prime

Signals governing thetrafficking and mis-trafficking of a ciliary GPCR, rhodopsin. Lodowski KH, Lee R, Ropelewski P, Nemet I,Tian G, Imanishi Y. J Neurosci. 2013 Aug 21;33(34):13621-38.

The mechanosensory structure of the hair cell requires clarin-1, a protein encoded by Ushersyndrome III causative gene.  Geng R,Melki S, Chen DH, Tian G, Furness DN, Oshima-Takago T, Neef J, Moser T, Askew C, Horwitz G, Holt JR, Imanishi Y,Alagramam KN. JNeurosci. 2012 Jul 11;32(28):9485-98.

Non-invasive multiphoton fluorescence microscopy resolves retinol and retinal condensation products in mouse eyes.  Palczewska G,Maeda T, Imanishi Y, Sun W, Chen Y,Williams DR, Piston DW, Maeda A, Palczewski K. Nat Med. 2010 Dec;16(12):1444-9.

Proteomic changes in the photoreceptor outer segment upon intense light exposure.  Hajkova D, Imanishi Y, Palamalai V, Rao KC, Yuan C, Sheng Q, Tang H, Zeng R, Darrow RM, Organisciak DT, Miyagi M.  J Proteome Res. 2010 Feb 5;9(2):1173-81.

Clarin-1, encoded by the Usher syndrome III causative gene, forms a membranous microdomain: Possible role of Clarin-1 inorganizing the actin cytoskeleton.  TianG, Zhou Y, Hajkova D, Miyagi M, Dinculescu A, Hauswirth WW, Palczewski K, GengR, Alagramam KN, Isosomppi J, Sankila EM, Flannery JG, Imanishi Y.  J Biol Chem. 2009 May 7.  doi:10.1074/jbc.M109.003160

Usher Syndrome IIIA Gene Clarin-1 is Essential for Hair Cell Function and AssociatedNeural Activation.  Geng R, Geller SF,Hayashi T, Ray CA, Reh TA, Bermingham-McDonogh O, Jones SM, Wright CG, Melki S,Imanishi Y, Palczewski K, AlagramamKN, Flannery JG.  Hum Mol Genet. 2009 May 3.  doi:10.1093/hmg/ddp210

Retinyl ester homeostasis in the adipose differentiation-related protein-deficient retina.  Imanishi Y*, Sun W, Maeda T, Maeda A, Palczewski K*.  J Biol Chem.2008 Sep 5;283(36):25091-102. (* corresponding authors)

RBP4 disrupts vitaminA uptake homeostasis in a STRA6-deficient animal model for Matthew-Woodsyndrome.  Isken A, Golczak M, OberhauserV, Hunzelmann S, Driever W, Imanishi Y,Palczewski K, von Lintig J.  Cell Metab. 2008 Mar;7(3):258-68. Featured on the cover

A Third Photoreceptor-Specific GRK Found in the Retina of Oryzias latipes (Japanese killifish).  Imanishi Y, Hisatomi O, Yamamoto S, Satoh T, Kotaka S, Kobayashi Y, Tokunaga F.  Zoolog Sci.2007;24(1):87-93.

Topology and membrane association of lecithin: retinol acyltransferase. Moise AR, Golczak M, ImanishiY, Palczewski K.  J Biol Chem. 2007 Jan 19;282(3):2081-90.

Retinoldehydrogenase (RDH12) protects photoreceptors from light-induced degeneration in mice. Maeda A, Maeda T, Imanishi Y, Sun W, Jastrzebska B, Hatala DA, Winkens HJ, Hofmann KP, Janssen JJ, Baehr W, Driessen CA, Palczewski K.  J Biol Chem. 2006 Dec 8;281(49):37697-704.

Effects of potent inhibitors of the retinoid cycle on visual function and photoreceptor protection from light damage in mice. Maeda A, Maeda T, Golczak M, Imanishi Y, Leahy P, Kubota R, Palczewski K. Mol Pharmacol. 2006 Oct;70(4):1220-9.

Autosomal recessive retinitis pigmentosa: E150K mutation in the opsin gene.  Zhu L, ImanishiY, Filipek S, Alekssev A, Jastrzebska B, Sun W, Saperstein DA, Palczewski K.  J Biol Chem. 2006 Aug4;281(31):22289-98.

Aberrant metabolitesin mouse models of congenital blinding diseases: formation and storage ofretinyl esters. Maeda A, Maeda T, Imanishi Y,Golczak M, Moise AR, Palczewski K.  Biochemistry. 2006 Apr 4;45(13):4210-9.

Pharmacologicaland rAAV Gene Therapy Rescue of Visual Functions in a Blind Mouse Model of Leber Congenital Amaurosis.  Batten ML, Imanishi Y, Tu DC, Doan T, Zhu L, PangJ, Glushakova L, Moise AR, Baehr W, Van Gelder RN, Hauswirth WW, Rieke F,Palczewski K.  PLoS Med. 2005 Nov;2(11):e333.

Lecithin:retinolacyl transferase is responsible for amidation of retinylamine, a potent inhibitor of the retinoid cycle.  GolczakM, Imanishi Y, Kuksa V, Maeda T, Kubota R, Palczewski K.  J Biol Chem.2005 Dec 23;280(51):42263-73.

Role of photoreceptor-specific retinol dehyrogenase (PRRDH) in the retinoid cycle in vivo.  Maeda A, Maeda T, Imanishi Y, Kuksa V, Alekseev A, Bronson DJ,Zhang H, Zhu L, Sun W, Saperstein DA, Rieke F, Baehr W, Palczewski K. J Biol Chem. 2005 May 13;280(19):18822-32.

Essential role of Ca2+-bindingprotein 4, a Cav1.4 channel regulator, for photoreceptor synaptic function.  Haeseleer F*, ImanishiY*, Maeda T, Possin DE, Maeda A, Lee A,Rieke F, and Palczewski K.  NatureNeuroscience. 2004; Oct;7(10):1079-87. (* authors contributed equally to the work) Recommended by F1000Prime

Identification ofall-trans-retinol: all-trans-13,14-dihydroretinol saturase.  MoiseAR, Kuksa V, Imanishi Y, Palczewski K.  J Biol Chem. 2004 Nov 26;279(48):50230-42. Featured on the cover

Hippocalcin in theolfactory epithelium: a mediator of second messenger signaling.  Mamman A, Simpson JP, Nighorn A, Imanishi Y, Palczewski K, Ronnett GV,and Moon C.  Biochem Biophys Res Commun.2004; 322: 1131-1139

Diversity ofguanylate cyclase-activating proteins (GCAPs) in teleost fish: Characterizationof three novel GCAPs (GCAP4, GCAP5, GCAP7 ) from zebrafish (Danio Rerio)and prediction of eight GCAPs (GCAP1-8) in pufferfish (Fugu Rubripes).  Imanishi Y*, Yang L*, Sokal I, Filipek S,Palczewski K, Baehr W.  J Mol Evol. 2004; 59: 204-217 (* authors contributed equally to the work)

Lecithin:retinolacyltransferase (LRAT) is essential for retention of retinyl esters in the eyeand in the liver.  Batten ML*, ImanishiY*, Maeda T*, Tu D, Moise AR, Bronson D, Possin D, Van Gelder RN, Baehr W, Palczewski K.  J Biol Chem. 2004 Mar 12;279(11):10422-32. (* authors contributed equally to the work)

Non-invasive two-photon imaging reveals retinyl ester storage structures in the eye.  ImanishiY, Batten ML, Piston DW, Baehr W, Palczewski K.  J Cell Biol. 2004 Feb 2;164(3):373-83. Featured on the cover and by “In This Issue of the journal

Retinoid cycle in thevertebrate retina: experimental approaches and mechanisms ofisomerization. Kuksa V, Imanishi Y, Batten M, Palczewski K, Moise AR.  Vision Res. 2003 Dec;43(28):2959-81.

Pharmacological chaperone-mediated in vivo folding and stabilization of the P23H-opsin mutant associated with autosomal dominant retinitis pigmentosa.  Noorwez SM, Kuksa V, Imanishi Y, Zhu L, Filipek S, Palczewski K, Kaushal S.  J Biol Chem. 2003 Apr 18;278(16):14442-50.

Dual-substrate specificity short chain retinol dehydrogenases from the vertebrate retina.  Haeseleer F*, Jang GF*, Imanishi Y*, Driessen CA, Matsumura M,Nelson P, Palczewski K.  J Biol Chem. 2002 Nov 22;277(47):45537-46. (* authors contributed equally to the work)

Characterization of retinal guanylate cyclase-activating protein 3 (GCAP3) from zebrafish to man. Imanishi Y, Li N, Sokal I, Sowa ME, Lichtarge O, Wensel TG, Saperstein DA, Baehr W, Palczewski K. Eur. J. Neurosci. 2002;15(1):63-78. Featured on the cover

Gene transfer mediated by recombinant baculovirus into mouse eye.  Haeseleer F, Imanishi Y, Saperstein DA, Palczewski K.  Invest Ophthalmol Vis Sci.2001;42(13):3294-300.

Two types ofarrestins expressed in medaka rod photoreceptors.  Imanishi Y, Hisatomi O, Tokunaga F.  FEBS Lett. 1999; 462:31-6.

Three kinds of guanylate cyclase expressed in medaka photoreceptor cells in both retina and pineal organ.  Hisatomi O, Honkawa H, Imanishi Y, Satoh T, Tokunaga F.  Biochem Biophys Res Comm. 1999;255:216-20.

A novel subtype of G-protein-coupled receptor kinase, GRK7, in teleost cone photoreceptors.  Hisatomi O, Matsuda S, Satoh T, Kotaka S, Imanishi Y, Tokunaga F.  FEBS Lett. 1998;424:159-64.

Arrestins expressed in killifish photoreceptor cells. Hisatomi O, Imanishi Y, Satoh T, Tokunaga F.  FEBS Lett.1997;411:12-18.

Review articles

Protein Sorting in Healthy and DiseasedPhotoreceptors. Imanishi Y. AnnuRev Vis Sci. 2019 Jun 21. doi: 10.1146/annurev-vision-091718-014843.

Spatiotemporalanalysis of protein transport and membrane morphogenesis in vertebratephotoreceptors. Imanishi Y. SeibutsuButsuri. 2016;56(1):018-22.

Applications of phototransformable fluorescent proteins for tracking the dynamics of cellular components. Nemet I, RopelewskiP, Imanishi Y. Photochem PhotobiolSci.2015 Sep 30;14(10):1787-806. doi: 10.1039/c5pp00174a. (Invited review article)

Two-Photon Microscopy: Shedding Light on the Chemistry of Vision.  Imanishi Y*, Lodowski KH, Koutalos Y*. Biochemistry. 2007 Aug 28;46(34):9674-84. (*corresponding authors)

Retinosomes: new insights into intracellular managing of hydrophobic substances in lipid bodies.  Imanishi Y, Gerke V, Palczewski K. J Cell Biol. 2004 Aug 16;166: 447-453. 

Rhodopsin phosphorylation: 30 years later.  MaedaT, Imanishi Y, Palczewski K.  Prog Retin Eye Res. 2003 Jul;22(4):417-34.

Calcium-binding proteins: intracellular sensors from the calmodulin superfamily.  Haeseleer F, Imanishi Y, Sokal I, Filipek S, Palczewski K.  

Book chapters

Rhodopsin Trafficking and Mistrafficking: Signals, Molecular Components, and Mechanisms. NemetI, Ropelewski P, Imanishi Y. Prog Mol Biol TranslSci. 2015;132:39-71. doi: 10.1016/bs.pmbts.2015.02.007.

Monitoring of rhodopsin trafficking and mistrafficking in live photoreceptors. Lodowski KH, Imanishi Y. Methods in molecularbiology (Clifton, N.J.). 2015; 1271:293-307.

Visualization of retinoid storage and trafficking by two-photon microscopy. Imanishi Y, Palczewski K.  Methods Mol Biol. 2010;652:247-61.

Evolution of visual pigments and related molecules. Tokunaga F, Hisatomi O, Satoh T, Taniguchi Y, Matsuda S, Imanishi Y, Honkawa H, Takahashi Y, Kobayashi Y, Yoshida M,Tsukahara Y. Novartis Found Symp. 1999;224:44-52; discussion 52-3. Review.

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